An international team of researchers has identified a stem group insect from the Late Mississippian period, about 324 million years ago. The discovery, published in Nature on August 26, offers new insight into how insects made the transition from water to land, suggesting a long amphibious phase rather than a sudden shift.

The research was led by Prof. Chenyang Cai of the Nanjing Institute of Geology and Paleontology of the Chinese Academy of Sciences (NIGPAS) and Erik Tihelka, a joint training Ph.D. student at the University of Cambridge. Collaborators from the United States, Spain, and other countries also contributed to the study.

A New Species from the Late Mississippian

The researchers describe a new species, Chosha praecursor, found in calcareous claystone concretions in the Tesnus Formation of the Marathon Uplift in western Texas. The specimens are adult female insects, not crustacean larvae as originally classified. Their bodies measure 32.09 millimeters long, and with the median caudal filament and two cerci, they reach a total length of 49.66 millimeters. These insects possessed an ovipositor, a terminal caudal filament, a segmented trunk, six walking legs, and abdominal appendages modified into paddle-like structures.

In addition to the new species, the researchers reevaluated three other enigmatic Paleozoic hexapods: Loverhulmia from the Early Devonian of Scotland and an unnamed hexapod from the Mazon Creek biota in Illinois, USA. They concluded that all three belonged to a primitive stem lineage of insects.

Filling the Gap in the Fossil Record

Undisputed hexapod body fossils first appear in the Early Devonian Rhynie Chert around 405 million years ago. However, clearly recognizable insect fossils do not become abundant until the Late Carboniferous, leaving an approximately 80-million-year gap. The discovery of C. praecursor, which sits in this gap, helps fill that void.

The findings suggest that insects did not become fully land-dwelling immediately. Instead, early forms retained and modified features inherited from aquatic ancestors, spending a prolonged period in an amphibious, semi-aquatic lifestyle. This supports the idea that an amphibious stage was a crucial bridge between fully aquatic and completely terrestrial environments.

The specimens provide direct fossil evidence that the evolution from water to land occurred gradually over an extended evolutionary process, challenging long-standing ideas about the insect body plan's development and offering a clearer picture of how pancrustacean ancestors transitioned onto land.